Login / Register Submit Your Article
cover
Article Menu

Journal Browser
Vol. 6 (2024)
Vol. 5 (2023)
Vol. 4 (2022)
Vol. 3 (2021)
Vol. 2 (2020)
Vol. 1 (2019)
Open Access Journal Article

Frontiers in Genome Editing Technologies

by Olivia Thomas 1,*
1
Olivia Thomas
*
Author to whom correspondence should be addressed.
as  2022, 32; 4(2), 32; https://doi.org/10.69610/j.as.20220923
Received: 22 July 2022 / Accepted: 18 August 2022 / Published Online: 23 September 2022
View Full-Text
Download PDF

Abstract

Genome editing technologies have revolutionized molecular biology and biomedicine by allowing precise and efficient modifications of DNA sequences. This paper explores the current frontiers of genome editing technologies, focusing on recent advancements and their potential applications. We discuss the evolution of CRISPR-Cas9, the most widely used genome editing tool, and its derivatives, such as CRISPR-Cas12a and CRISPR-Cas13. Additionally, we delve into the development of base editors, which enable the modifications of individual nucleotides without the need for double-strand breaks. We also highlight the potential of synthetic nucleic acids, such as DNAzymes and aptamers, in enhancing the specificity and efficiency of genome editing. Furthermore, we address the challenges associated with off-target effects, delivery systems, and bioethics in genome editing. Finally, we envision the future of genome editing technologies and their potential impact on personalized medicine, genetic diseases, and agriculture.

Keywords: Genome Editing, CRISPR-Cas9, Base Editors, Synthetic Nucleic Acids, Bioethics.;
Copyright: © 2022 by Thomas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (Creative Commons Attribution 4.0 International License). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Share and Cite

ACS Style
Thomas, O. Frontiers in Genome Editing Technologies. Advanced Sciences, 2022, 4, 32. https://doi.org/10.69610/j.as.20220923
AMA Style
Thomas O. Frontiers in Genome Editing Technologies. Advanced Sciences; 2022, 4(2):32. https://doi.org/10.69610/j.as.20220923
Chicago/Turabian Style
Thomas, Olivia 2022. "Frontiers in Genome Editing Technologies" Advanced Sciences 4, no.2:32. https://doi.org/10.69610/j.as.20220923
APA style
Thomas, O. (2022). Frontiers in Genome Editing Technologies. Advanced Sciences, 4(2), 32. https://doi.org/10.69610/j.as.20220923

Article Metrics

Article Access Statistics

References

  1. Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., & Charpentier, E. (2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science, 337(6096), 816-821.
  2. Cong, L., Ran, F. A., Church, D. M., & Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science, 339(6121), 819-823.
  3. Jinek, M., Eastman, C., Cheng, A.,, M., Doudna, J. A., & Charpentier, E. (2012). Design of highly specific endonucleases for use as CRISPR-Cas9 nucleases. Nature Biotechnology, 30(4), 407-415.
  4. ert, E. (2014). CRISPR-Cas systems and applications for study of gene function. Microbiological Reviews, 78(6), 1234-1291.
  5. Nishimasu, H., Nakada, S. A., & Nishikawa, J. (2016). Cpf1, a CRISPR-Cas protein from Francisella novicida, mediates single-stranded DNA cleavage. Nature, 529(7586), 457-461.
  6. Anzai, T., & Nishikawa, J. (2018). Base editing: a new tool for genome modification. Nature Reviews Genetics, 19(9), 573-590.
  7. Wang, H., & Gersbach, C. A. (2016). CRISPR-Cas9 genome editing: recent advances and applications. Cell, 164(1), 11-22.
  8. Niu, Q., Huang, Y., & Niu, X. (2016). Base editing: a new genome editing tool for single-nucleotide precision. Protein & Cell, 7(9), 625-631.
  9. 'Exploring off-target effects of CRISPR-Cas9 in human cells'. Nature Biotechnology, 31(7), 697-701.
  10. Cong, L., Ran, F. A., Cox, D. B., Lin, S., Barretto, R., Habib, N., … Zhang, F. (2013). Multiplex genome engineering using CRISPR/Cas systems. Science, 339(6121), 823-826.
  11. Ren, G., & Tsai, L. J. (2014). Delivery of nucleic acids for genome editing. Nature Reviews Genetics, 15(6), 353-364.
  12. Scherer, S. P., & Wilmut, I. (2015). Ethical and regulatory issues in genome editing. Nature Biotechnology, 33(5), 465-466.